Journal: Mass Spectrometry

Article Title: High-Temperature Liquid Chromatography and the Hyphenation with Mass Spectrometry Using High-Pressure Electrospray Ionization

doi: 10.5702/massspectrometry.S0079

Figure Lengend Snippet: Fig. 1. Implementation of HTLC using a conventional atmospheric pressure ESI source for >100 μL/min operation. The heated column is connected to the ESI source via a fused silica capillary with 50 μm inner diameter that serves as a flow restrictor.

Article Snippet: The ESI emitter (stainless steel capillary of 0.1 mm i.d. and 0.2 mm o.d. from Nilaco, Tokyo, Japan) was housed inside an ion source chamber made of aluminum alloy.

Techniques:

Journal: Mass Spectrometry

Article Title: High-Temperature Liquid Chromatography and the Hyphenation with Mass Spectrometry Using High-Pressure Electrospray Ionization

doi: 10.5702/massspectrometry.S0079

Figure Lengend Snippet: Fig. 4. Schematic of the hyphenation of high-temperature capillary LC with high-pressure ESI-MS. The ion source is pressurized with air from a compressor. The fused silica tube from the injector is connected directly to the analytical column.

Article Snippet: The ESI emitter (stainless steel capillary of 0.1 mm i.d. and 0.2 mm o.d. from Nilaco, Tokyo, Japan) was housed inside an ion source chamber made of aluminum alloy.

Techniques:

Journal: Mass Spectrometry

Article Title: High-Temperature Liquid Chromatography and the Hyphenation with Mass Spectrometry Using High-Pressure Electrospray Ionization

doi: 10.5702/massspectrometry.S0079

Figure Lengend Snippet: Fig. 6. Photograph showing the appearance of bubbles from the ESI capillary. The ESI is at near room temperature while the column is kept at 100°C. The cavitation stops when the ambient around the ESI emitter is pressurized to >1 atm.

Article Snippet: The ESI emitter (stainless steel capillary of 0.1 mm i.d. and 0.2 mm o.d. from Nilaco, Tokyo, Japan) was housed inside an ion source chamber made of aluminum alloy.

Techniques:

Journal: Mass Spectrometry

Article Title: High-Temperature Liquid Chromatography and the Hyphenation with Mass Spectrometry Using High-Pressure Electrospray Ionization

doi: 10.5702/massspectrometry.S0079

Figure Lengend Snippet: Fig. 8. Detection of 100 fmol methamphetamines in diluted raw urine by a) high-temperature capillary LC-MS (column i.d.: 0.1 mm) using HPESI under an operating pressure of 0.4 MPa, and b) high-temperature LC-MS (column i.d.: 1 mm) using pneumatically assisted ESI under atmospheric pressure. Column temperatures: 140°C, mobile phase: 9% (w/w) acetonitrile aqueous solution, solution flow rates: 10 μL/min for HPESI (a) and 400 μL/min for pneumatically assisted ESI (b). Insets show the EIC for the protonated methamphetamine. Reproduced with permission from Analyst (2018), 143, 5552–5558. )

Article Snippet: The ESI emitter (stainless steel capillary of 0.1 mm i.d. and 0.2 mm o.d. from Nilaco, Tokyo, Japan) was housed inside an ion source chamber made of aluminum alloy.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Mass Spectrometry

Article Title: High-Temperature Liquid Chromatography and the Hyphenation with Mass Spectrometry Using High-Pressure Electrospray Ionization

doi: 10.5702/massspectrometry.S0079

Figure Lengend Snippet: Fig. 9. High-pressure ESI mass spectra of bovine ubiquitin in 2% v/v formic acid under different solution flow rates. a) 10 μL/min, b) 4 μL/min, and c) 2 μL/min. ESI capillary temperate is 220°C for all cases. The corresponding residence time of solution within the heated capillary are 1.4 s (a), 3.5 s (b), and 7 s (c). Asterisk * depicts the peak from intact ubiquitin. Reproduced with permission from J. Mass Spectrom. (2016), 51, 396–411. )

Article Snippet: The ESI emitter (stainless steel capillary of 0.1 mm i.d. and 0.2 mm o.d. from Nilaco, Tokyo, Japan) was housed inside an ion source chamber made of aluminum alloy.

Techniques: Ubiquitin Proteomics